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Superoxide design is actually detected inside blood and you can BALF utilizing the CMH twist probe. Blood or BALF examples had been accumulated out of PBS- and you can Bleo-addressed mice and you will incubated instantly that have CMH. The brand new trials was moved to the new PTFE tubing and you will thumb suspended, and you may EPR research is accumulated from the 77 K. The new concentration of nitroxide (CM.) obtained in the bloodstream incubated which have CMH (0.dos mM) at the 37 degrees to own ten minute (Figure 4A).

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